The Effects of Deprenylonsynaptic Zone after Spinal Cord Injury in Rats

In order to distinguish spinal cord injury (SCI) caused by compression model, we demonstrated ultrastructural and morphometric changes in synaptic lesion after SCI. 72 female Sprague Dawley rats weighining 250-300g, were randomly divided into four groups (N =18). The sham groups were only subjected to laminectomy. All administered 2.5 mg/kg Deprenyl (CIPLA/ India, dissolved in 1cc saline) or equal volume of saline. At the time of operating for sham or SCI surgery groups daily injection were done and continued till they were sacricfied: group A: SCI + Deprenyl, group B: SCI + vehicle, group C: Sham + Deprenyl, and group D: Sham + vehicle. In every group, after 1, 2, 4weeks 6 animals were sacrificed. SCI caused motoneuron decreased in Anterior horn of spinal cord with hole and hemorrhage. Deprenyl decreased this process (P≤ 0.05). The most pattern of synaptophysin in group A which received Deprenyl, was cytoplasmic (P≤ 0.05). Synaptic and neuronal mitochondria changes were analyzed in a blinded manner for qualitative ultrastructural changes. By SCI, pathological changes including irregularity of the synaptic membrane and synaptic cleft with displacement of synaptic vesicles and irregularity of mitochondria were seen beside synaptophysin reduction. Deprenyl decreased pathological changes and maintained motoneurons after SCI.